ࡱ> vxu_ bjbj .bb(((((<<<<,h<P$HX((( ((rɃ 0PRRR( PR : ANNEX III INFORMATION TO IBSC/ RCGM TO CARRY OUT RESEARCH FOR DEVELOPMENT OF TRANSGENIC PLANTS Application for clearance by IBSC/ RCGM to carry out research! experimental trials involving transgenic plants in green house and in contained open fields. 1.Name of the ApplicantDesignation(a) Address (Registered Office)Telephone No.Telex No.Fax No.e-mail(b) Address (Research Station)Telephone No.Telex No.Fax No.e-mail2.Application for :2.1Purpose2.2NewYesNo2.3Ongoing ProjectYesNoIf yes, No. & Date of LOI issued :No. & Date of Permit issued :2.4If yes, briefly state the purpose for which permission was granted. 2.5Category of experiments as per the Guidelines of DBT3.Objectives of the proposal 4.Description of the transgenic plants employed in the research proposal (in scientific terms)(for new application only)4.1Description of transgenic plants4.2Anticipated new characters in transgenic plants/ Expected difference in the transgenic plants compared to non-transgenic counterparts.4.3Description of the target gene and mode of action.5.Details on(Nucleic acid(s) & Vectors) :5.1Source of nucleic acid(s) :5.2Copy number of gene(s) :5.3Nucleic acid sequence (Please enclose the nucleic acid sequence map of the target gene) :5.4Vector(s) description (please enclose the Plasmid map) :6.Summary of the proposed work plan utilizing plant parts/ transgenic plants (please check it from the follollowing areas and provide the detailed work plan).6.1Basic transformation work and tissue culture procedure at laboratory level and standardize the procedure to assess the expression of the target gene in the transformed material.6.2Transfer of target gene imported/ indigenously isolated in Indian germplasm and to assess the expression of the target gene in the transformed material.6.3Transfer of target gene from imported seed materials to indigenous species and to assess the expression of the target gene in the transformed material.6.4Green house experiments on transgenic plants for testing/ assessing the efficacy of their new characters.6.5Assessment of toxicity and allergenicity of transgenic seeds, plants and plant parts (if yes, please provide information on the following whichever is applicable)i) Description of the material (leaf, seeds, fruits etc.) used for testing.ii) Biochemical characterization of the material in termS of near equivalence to its non-transgenic counterpart.iii) Information on quantity of target gene product in different parts of the plant at different stages of development.iv) Toxicity and Allerge.nicity protocols and the address of the lab where these studies are proposed to be conducted.v) Institutional Animal Ethics CQmmittee's Approval.vi) Effect of target gene on soil microflora and nematodes.6.6Contained open field trials (if yes, please provide information on the following)i) Experimental field design along with plot plan.ii) Location map indicating the experimental plot.iii) Ownership of the experimental plot (Applicants own plot/ leased plot Agricultural University plot/ ICAR owned plot/ others).iv) Agreement signed for using experimental plot (wherever applicable).v) Containment description in the region of the experimental plot.vi) Experimental area under transgenic plants at each location and the tot area.vii) Normal cropping season of the plant variety.viii) Crop season in which experiments are proposed.7.Site/ Location of the research work/ experimental field :8.Decontamination and disposal mechanisms9.Risk management (Emergency plan) 10.Any other relevant information11.Declaration:I declare that the information provided in the above format is correct and accurate to the best of my knowleage. The "Recombinant DNA Safety Guidelines 1990 and Revised Guide1ines for research in transgenic plants & Guidelines for Toxicity and Allergenicity Evaluation of transgenic seeds, plants and plant parts" 1998 brought out by the Department of Biotechnology, Ministry of Science & Technology, Govt. of India will be and is being strictly followed. The imported material will be and is being utilized for the said purpose only. 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